Browsing by Subject "Canny's operator"

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Access status: Open Access ,
Fine-tuned spot detection on ELISPOT images
(Wydawnictwa AGH, 2009) Bieniecki, Wojciech; González Martinez, Victor Manuel; Grabowski, Szymon
Enzyme linked immunospot assay (ELISPOT) is a powerful technique used for detection and quantification of antigen specific immunological responses at the single cell level, which could have a prognostic value to diagnose the long-term graft outcome (i.e., kidney) and to evaluate the level of immunosuppression therapy needed. Basically, ELISPOT image contains round spots of different color, intensity and size. The image analysis should include accurate measurements of spot properties, like area and color. We propose a new approach to spot detection and measurement on ELISPOT microscope images, combining the Canny operator and a circle-fitting routine that we previously used (in somewhat different context) for that class of images. Good edge detection algorithms, like the Canny one, yield locally accurate spot contour arcs but their drawback is that the found arcs are not connected, i.e., do not constitute full contours. We solved the problem, fitting arcs belonging to the same spot contour. For each arc, a circle to each it belongs was approximated and then circle clus-tering was performed not to produce too many (false) contours.