Browsing by Subject "calcium phosphate"

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Access status: Open Access ,
Calcium phosphate-based hybrid biomaterials for bone regeneration and local antibacterial therapy
Pańtak, Piotr; Belcarz, Anna; Czechowska, Joanna P.; Zima, Aneta
Wydział Inżynierii Materiałowej i Ceramiki
Composites based on hydroxyapatite (HA) and/or tricalcium phosphate (TCP) along with polymers, are widely used in bone regeneration due to their excellent biocompatibility. However, one of the challenges associated with their implantation procedures is the occurrence of inter- and post-operative bacterial infections. To address this issue biomaterials can be enriched with antibacterial agents such as: metallic nanoparticles (eg. AgNPs, AuNPs), ions (Cu2+, Ag+) or antibiotics, which can effectively inhibit the growth and proliferation of a wide range of bacteria. In this study, composites based on αTCP and hybrid granules were developed and examined. Hybrid granules composed of hydroxyapatite, methylcellulose and/or chitosan were obtained via wet-chemical method. Materials were enriched with various nanoparticles, including AuNPs, AgNPs and CuONPs. The chemical and phase composition (XRF,XRD), setting times (Gillmore Apparatus), and compressive strength (Instron 3345) were examined. Microstructure was observed using SEM. Furthermore, the bioactive potential in vitro in SBF as well as antibacterial activity against E. coli, and S. aureus were evaluated. In the present study, some key features were improved by developing novel biomaterials. The designed composites offer biocompatibility, sufficient mechanical and antibacterial properties. Based on the obtained results, it can be concluded that the developed materials hold promise as candidates preventing post-surgical infections in hard tissue applications. Further biological studies are necessary to comprehensively characterize these biomaterials. Acknowledgments Research project supported by the programme ‘Excellence initiative – research university” for the AGH University of Science and Technology and the AGH Faculty of Materials Science and Ceramics (Project No. 16.16.160.557). This research was funded in whole or in part by National Science Centre, Poland (Project MINIATURA7 No. 2023/07/X/ST11/00705). For the purpose of Open Access, the author has applied a CC-BY public copyright licence to any Author Accepted Manuscript (AAM) version arising from this submission.
Access status: Open Access ,
Hybrid bone substitute containing tricalcium phosphate and silver modified hydroxyapatite–methylcellulose granules
(2024) Czechowska, Joanna P.; Dorner-Reisel, Annett; Zima, Aneta
Wydział Inżynierii Materiałowej i Ceramiki
Despite years of extensive research, achieving the optimal properties for calcium phosphate-based biomaterials remains an ongoing challenge. Recently, ‘biomicroconcretes’ systems consisting of setting-phase-forming bone cement matrix and aggregates (granules/microspheres) have been developed and studied. However, further investigations are necessary to clarify the complex interplay between the synthesis, structure, and properties of these materials. This article focusses on the development and potential applications of hybrid biomaterials based on alpha-tricalcium phosphate (αTCP), hydroxyapatite (HA) and methylcellulose (MC) modified with silver (0.1 wt.% or 1.0 wt.%). The study presents the synthesis and characterization of silver-modified hybrid granules and seeks to determine the possibility and efficiency of incorporating these hybrid granules into αTCP-based biomicroconcretes. The αTCP and hydroxyapatite provide structural integrity and osteoconductivity, the presence of silver imparts antimicrobial properties, and MC allows for the self-assembling of granules. This combination creates an ideal environment for bone regeneration, while it potentially may prevent bacterial colonization and infection. The material’s chemical and phase composition, setting times, compressive strength, microstructure, chemical stability, and bioactive potential in simulated body fluid are systematically investigated. The results of the setting time measurements showed that both the size and the composition of granules (especially the hybrid nature) have an impact on the setting process of biomicroconcretes. The addition of silver resulted in prolonged setting times compared to the unmodified materials. Developed biomicroconcretes, despite exhibiting lower compressive strength compared to traditional calcium phosphate cements, fall within the range of human cancellous bone and demonstrate chemical stability and bioactive potential, indicating their suitability for bone substitution and regeneration. Further in vitro studies and in vivo assessments are needed to check the potential of these biomaterials in clinical applications.