Browsing by Subject "differentiation"

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Access status: Open Access ,
Controlled therapeutic cholesterol delivery to cells for the proliferation and differentiation of keratinocytes
(2024) Moradi, Ahmadreza; Lichawska-Cieslar, Agata; Szukala, Weronika; Jura, Jolanta; Berniak, Krzysztof; Stachewicz, Urszula
Wydział Inżynierii Metali i Informatyki Przemysłowej
The challenge of enhancing wound healing and skin regeneration, particularly in conditions like burns and diabetic wounds, necessitates innovative solutions. Cholesterol, often associated with cardiovascular diseases, plays vital roles in cellular functions, maintaining skin integrity and preserving the skin barrier. Here, we explore cholesterol's significance, its influence on keratinocytes, and its potential application in skin regeneration. The study utilizes electrospun polyimide (PI) fibers as a cholesterol carrier model and investigates its impact on HaCaT keratinocytes, marking the first time tracked cholesterol delivery from the scaffold into cells. We demonstrate that an optimal concentration of 0.7 mM cholesterol in the medium enhances cell proliferation, while higher concentrations have negative effects. Cholesterol-enriched scaffolds significantly increase cell proliferation and replicative activity, especially in a 3D culture environment. Moreover, cholesterol influences keratinocyte differentiation, promoting early differentiation while inhibiting late differentiation. These findings suggest that cholesterol-loaded scaffolds can have applications in wound healing by promoting cell growth, regulating differentiation, and potentially accelerating wound closure. Further research in this area will lead to innovative wound management and tissue regeneration strategies.
Access status: Restricted ,
Petrography, mineral chemistry and modeling of time scales of magmatic processes at the Nola Seamount, Cape Verde Islands
(Data obrony: 2012-07-16) Kohut, Marzena
Wydział Geologii, Geofizyki i Ochrony Środowiska
Cape Verde Islands are well known example of hot spot activity, but still not fully understood. The archipelago is located in the Central – Atlantic. The Cape Verde submarine plateau was sampled during the Meteor M80/3 research cruise in January 2010. An extensive suite of submarine lavas were collected by dredging (DR) and remotely operated vehicle (ROV). Detailed petrography and mineral chemistry of the Nola Seamount, located in the northwest of the Cape Verde archipelago is presented with the aim to unravel the pre-eruptive processes, and hence contribute to our understanding of magma ascent and evolution in the Cape Verde. The sampled volcanics are basanites and ankaramites. All samples comprise a glassy to microcrystalline groundmass that hosts phenocrysts and microphenocrysts of mainly euhedral and subhedral clinopyroxenes and euhedral olivines. Clinopyroxene crystals are the dominant phenocryst phase and they exhibit strong zonation with optically distinct cores of variable composition with increasing FeO, TiO2 and Al2O3 and decreasing MgO content from core to rim of the crystals. Moreover, three geochemically different groups of clinopyroxenes can be distinguish, the most primitive with high Mg low Al2O3 contents, a group with intermediate composition, and the most evolved group, with low Mg and high Al2O3. The high Al contents of the rims suggest formation during decompression. Based on high resolution BSE images and geothermobarometry data, diffusional smoothing of Fe–Mg compositional gradients in clinopyroxene crystals is used to model the pre-eruption residence times of crystals at magmatic temperatures. We obtained three timescales: 800 – 1000 years, 200 – 400 years, <100 years, which correlate with the compositional variations of Mg and Al contents, and thus allow to trace magma ascent in considerable detail.