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Browsing by Subject "proteomika"

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  • thumbnail.journal.alt
    Item type:Thesis, Access status: Restricted ,
    Analiza ilościowa białek z wykorzystaniem znakowania izobarycznego
    (Data obrony: 2020-09-30) Mazurek, Martyna
    Wydział Inżynierii Materiałowej i Ceramiki
  • thumbnail.journal.alt
    Item type:Thesis, Access status: Restricted ,
    Glikoproteom wybranych struktur ośrodkowego układu nerwowego
    (Data obrony: 2017-07-14) Juszczyk, Dorota
    Wydział Elektrotechniki, Automatyki, Informatyki i Inżynierii Biomedycznej
  • thumbnail.journal.alt
    Item type:Thesis, Access status: Restricted ,
    Opracowanie metody oznaczania poziomu białka XRCC5 w próbkach guza raka żołądka
    (Data obrony: 2019-01-22) Żołynia, Anna
    Wydział Elektrotechniki, Automatyki, Informatyki i Inżynierii Biomedycznej
  • thumbnail.journal.alt
    Item type:Thesis, Access status: Restricted ,
    Optymalizacja metod znakowania fibroblastów na potrzeby badań proteomicznych.
    (Data obrony: 2018-10-09) Murawska, Kamila
    Wydział Fizyki i Informatyki Stosowanej
  • thumbnail.journal.alt
    Item type:Thesis, Access status: Restricted ,
    Optymalizacja metody analizy proteomu z zastosowaniem kolumny MudPit
    (Data obrony: 2018-01-22) Skalska, Agata
    Wydział Elektrotechniki, Automatyki, Informatyki i Inżynierii Biomedycznej
  • thumbnail.journal.alt
    Item type:Thesis, Access status: Restricted ,
    Oznaczenie białek charakterystycznych dla tkanki raka żołądka metodą 2DnanoLC-MS/MS.
    (Data obrony: 2018-06-25) Sas, Karolina
    Wydział Inżynierii Materiałowej i Ceramiki
  • thumbnail.journal.alt
    Item type:Book, Access status: Restricted ,
    Proteomika i metabolomika
    (Wydawnictwa Uniwersytetu Warszawskiego, ©© 2010) Kraj, Agnieszka; Drabik, Anna; Silberring, Jerzy
  • thumbnail.journal.alt
    Item type:Doctoral Dissertation, Access status: Restricted ,
    Quantitative analysis of phosphoproteome of the selected brain structures in morphine dependence
    (2010-06-18) (Data obrony: 2013) Sucharski, Filip
    Wydział Inżynierii Materiałowej i Ceramiki
    The aim of the project was the search for, and identification of the markers of drug dependence. The research was focused on the identification of molecular mechanisms involved in these processes and clarification of the role of particular proteins in morphine dependence. Project puts special emphasis on phosphoproteins, and in general on phosphorylation, as a main mechanism involved in control of essentially any biological process and many diseases. High dynamism and reversible character, make phosphorylation a main regulator of signaling networks. Therefore, a global wide-scale analysis of phosphoproteome however, troublesome, gives hope on detailed recognition of complicated molecular mechanisms and mutual connections between metabolic pathways. It is known, that drug dependence has a huge impact on many cell functions as well as biological processes, for which the relation with the drug dependence seems to be indirect. Thus, only a global analysis of a state of phosphoproteins, in particular kinases with their mutual relations, can contribute to solving a puzzle. The project involves the newest, fully quantitative, methods for phosphoproteome analysis. In particular a special work-flow based on two methods of phosphopeptides enrichment (TiO2, IMAC), data- dependent mass spectrometry acquisition and specific bioinformatic platform were developed. This approach allowed for simultaneous analysis of CID/ETD data from the Bruker ion-trap mass spectrometer with Mascot as identification tool and finally with the Trans-proteomic pipeline. This work describes methods for phosphoproteomics and gives a detailed protocols for sample preparation, phosphopeptides enrichment and mass spectrometry analysis. However it puts special emphasis on data analysis including self-programmed python scripts for data conversion, which is crucial in such wide-scale experiments. The project resulted in identification and quantitation of a large number of phosphoproteins, which may be considered as potential biomarkers for morphine dependence. A few of them were discussed in details, however further research, especially based on MRM (Multiple reaction monitoring) approach is required.
  • thumbnail.journal.alt
    Item type:Thesis, Access status: Restricted ,
    Wykorzystanie technik MS i LC-MS do analiz proteomu w układach biologicznych
    (Data obrony: 2020-06-30) Bork, Magdalena
    Wydział Inżynierii Materiałowej i Ceramiki
  • thumbnail.journal.alt
    Item type:Thesis, Access status: Restricted ,
    Zastosowanie techniki ProteoMiner w celu identyfikacji cytochromów P450 we frakcji mikrosomalnej wątroby
    (Data obrony: 2017-07-14) Machnik, Nina
    Wydział Elektrotechniki, Automatyki, Informatyki i Inżynierii Biomedycznej