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Browsing by Subject "region boundaries"

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    Item type:Article, Access status: Open Access ,
    Algorytm segmentacji mikroskopowych obrazów okrzemek w preparatach z zanieczyszczeniami osadowymi
    (Wydawnictwa AGH, 2009) Sekulska-Nalewajko, Joanna; Gocławski, Jarosław
    In the paper a new, robust to artefacts, method of microscopic diatom image segmentation has been presented. Images are acquired in grey-levels using bright field microscopy from specimens with impurities such as dust specks, debris or sand crystals. The method assumes superposition of images from different focal planes including diatom surface ornamentation and boundaries. Diatom object contours are detected using Canny filtering and their background regions are extracted independently applying bottom hat filtering and morphological reconstruction. Contour gaps are filled by linking of all contour ends inside of individual diatom background regions. To distinguish regularly shaped objects of diatoms from artefacts, contour curvatures, symmetry axes and centres are verified for each segmented object. Directional ornamentation of diatom frastules (if present) is detected by histogram analysis of phase images inside of individual region masks.