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Browsing by Subject "rozkład dna"

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    Item type:Article, Access status: Open Access ,
    Metoda segmentacji obrazów jąder komórkowych roślin w cytometrii DNA z heterogenicznym rozkładem wewnątrz jądra
    (Wydawnictwa AGH, 2006) Gocławski, Jarosław; Anioł, Patryk
    The paper presents an algorithm of plant nuclei image segmentation preceding the procedure of DNA contents measurement. It is based on iterative multilevel thresholding of grey-level differential image, obtained by preliminary subtraction of components in RGB color space. The subtraction reduces non-stained background artifacts. Splitting of merged cell nuclei has been provided using shape-based watershed method referred to the distance transform of the objects. It has been shown, that multilevel thresholding plays a key role in the proper discrimination of nuclei profiles with highly nonuniform DNA distribution.